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Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes
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Bioquímica
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JoVE Journal Bioquímica
Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

DOI:
10.3791/61580-v

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07:38 min

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September 01, 2020

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,
1Department of Biomolecular Sciences,University of Mississippi, 2Department of Chemistry and Biochemistry,University of Mississippi, 3GenNext Technologies, Inc.

Capítulos

  • 00:04Introduction
  • 00:47Fast Photochemical Oxidations of Proteins (FPOP) Optical Bench Preparation
  • 02:43FPOP Experiment
  • 03:58Compensation Performance
  • 05:45Results: Representative Adalimumab FPOP and Compensated Myoglobin FPOP in MES Buffer
  • 06:51Conclusion

Summary

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Tadução automática

Fast photochemical oxidation of proteins is an emerging technique for the structural characterization of proteins. Different solvent additives and ligands have varied hydroxyl radical scavenging properties. To compare the protein structure in different conditions, real-time compensation of hydroxyl radicals generated in the reaction is required to normalize reaction conditions.

Tags

Keywords: Real-time CompensationFast Photochemical OxidationsProtein Topography ChangesProtein ConfirmationProtein InteractionsBuffer CompositionSample PurityProtein ConcentrationDosimetry MethodDrug DevelopmentProtein StructureFPOP Optical BenchLaserInline DosimeterExcimer LaserMotorized StageHydrogen PeroxideFPOP Solution

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