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全鼠眼的消化，用于单核噬细胞的多参数流细胞学分析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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全鼠眼的消化，用于单核噬细胞的多参数流细胞学分析

4,695 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

4 Views
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1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Tags

DigestionMouse EyesMononuclear PhagocytesFlow CytometryMacrophagesMonocytesMicrogliaDendritic CellsCell Surface MarkersChoroidal NeovascularizationDiabetic RetinopathyExperimental Autoimmune UveitisLupusCytometer ConfigurationDissectionMechanical Disruption

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Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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