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Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber
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Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

DOI:
10.3791/61234-v

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09:07 min

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June 11, 2020

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, ,
1Department of Experimental Medical Science,Lund University, 2Lund University Diabetes Centre

Capítulos

  • 00:04Introduction
  • 01:08Transplantation Procedure
  • 02:45Image Analysis
  • 06:58Results: Image Segmentation of a Human Islet Grafted in the ACE of a NOD.ROSA-tomato.Rag2-/- Recipient Mouse
  • 08:17Conclusion

Summary

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The goal of this protocol is to continuously monitor the dynamics of the human pancreatic islet engraftment process and the contributing host versus donor cells. This is accomplished by transplanting human islets into the anterior chamber of the eye (ACE) of an NOD.(Cg)-Gt(ROSA)26Sortm4-Rag2-/-mouse recipient followed by repeated 2-photon imaging.

Tags

Keywords: In Vivo ImagingPancreatic Islet GraftingAnterior Eye ChamberBeta Cell Replacement TherapyIslet TransplantationTwo-photon MicroscopyIslet Function And ViabilityDiabetic ComplicationsReal-time Imaging

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