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Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro
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JoVE Journal Neurociência
Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

DOI:
10.3791/61190-v

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08:01 min

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June 02, 2020

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,
1Wuhan Center for Disease Control and Prevention, 2College of Life Science and Health,Wuhan University of Science and Technology

Capítulos

  • 00:05Introduction
  • 00:49Differentiation from mESCs to NPCs (Phase I)
  • 04:40Differentiation from NPCs to neurons (Phase II)
  • 05:32Results: Optimal Protocols for Phase I and Phase II Differentiation
  • 07:07Conclusion

Summary

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Tadução automática

Here, we established a low cost and easy to operate method that directs fast and efficient differentiation from embryonic stem cells into neurons. This method is suitable for popularization among laboratories and can be a useful tool for neurological research.

Tags

Neuronal DifferentiationMouse Embryonic Stem CellsIn VitroEmbryoid Body FormationRetinoic Acid InductionNeurogenesisNeural LineageStem Cell Differentiation

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