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在存在治疗性抗体的情况下,由FcγRIIIa参与驱动的人类自然杀伤细胞事件的评估
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
DOI:

09:54 min

May 22, 2020

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Capítulos

  • 00:05Introduction
  • 00:27Antibody-Mediated Activation of NK Cells
  • 03:12Interrogation of Signaling Molecules by Western Blot Analysis
  • 03:47Isolation of mRNA and Preparation for Chemokine/Cytokine Assessment
  • 04:39Cytoskeletal Rearrangement Assessment
  • 05:57Assessing Degranulation Using CD107a Surface Staining
  • 07:10Results: Downstream Applications of NK Cell Activation Assay
  • 09:20Conclusion

Summary

Tadução automática

本文描述了通过人类自然杀伤细胞中的治疗性抗体研究FcγRIIIa驱动事件的方案。该人工刺激平台允许询问下游效应器功能,例如脱颗粒,趋化因子/细胞因子产生以及由参与结合的抗体的FcγRIIIa和Fc部分介导的信号通路。

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