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卡诺哈布迪蒂斯精英热休克反应的测量诱导标准化方法
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
DOI:

06:01 min

July 03, 2020

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Capítulos

  • 00:04Introduction
  • 00:54Fluorescent Imaging of HSR Reporters
  • 02:58Thermorecovery Assay
  • 03:32Results: HSR Induction Analysis
  • 05:17Conclusion

Summary

Tadução automática

在这里,提出了标准化的协议,以评估在卡诺哈布迪蒂斯埃利根的热休克反应(HSR)的诱导使用RT-qPCR在分子水平,荧光报告器在细胞水平,并在有机体水平的光覆盖。

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