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Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

DOI:
10.3791/61030-v

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06:01 min

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July 03, 2020

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, , ,
1Department of Biomedical and Chemical Engineering and Sciences,Florida Institute of Technology

Capítulos

  • 00:04Introduction
  • 00:54Fluorescent Imaging of HSR Reporters
  • 02:58Thermorecovery Assay
  • 03:32Results: HSR Induction Analysis
  • 05:17Conclusion

Summary

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Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.

Tags

Heat Shock ResponseCaenorhabditis ElegansThermal ToleranceThermal RecoverySynchronized Worm PopulationDevelopmental TimingHeat ShockGFP SynthesisAgarose PadLevamisole

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