Journal
/
Imunologia e Infecção
/
Bestämning av kemisk hämmare effektivitet mot intracellulära Toxoplasma Gondii tillväxt med hjälp av en Luciferase-baserad tillväxt analys
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
Please note that all translations are automatically generated. Click here for the English version.

Bestämning av kemisk hämmare effektivitet mot intracellulära Toxoplasma Gondii tillväxt med hjälp av en Luciferase-baserad tillväxt analys

6,879 Views

•

09:49 min

•

April 29, 2020

DOI:

10.3791/60985-v

DOI:
10.3791/60985-v

•

09:49 min
April 29, 2020

•

1 Views
, , , , ,
1Department of Biological Sciences,Clemson University, 2Eukaryotic Pathogens Innovation Center,Clemson University

Tags

Toxoplasma GondiiLuciferase-based Growth AssayChemical InhibitorDrug TargetCRISPR-Cas9Intracellular PathogenHigh-throughput ScreeningParasite Doubling TimeHuman Foreskin FibroblastsLuciferase SubstrateMicroplate ReaderNormalized Fold-changeLinear Regression

Read Article

Cite this Article

Key, M., Bergmann, A., Micchelli, C., Thornton, L. B., Millard, S., Dou, Z. Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay. J. Vis. Exp. (158), e60985, doi:10.3791/60985 (2020).

Download .ris file

Copy

Share Video

.

Copy