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Luciferase Tabanlı Büyüme Testini Kullanarak Hücre İçi Toksoplazma Gondii Büyümesine Karşı Kimyasal Inhibitör Verimliliğin Belirlenmesi
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JoVE Journal Imunologia e Infecção
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
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Luciferase Tabanlı Büyüme Testini Kullanarak Hücre İçi Toksoplazma Gondii Büyümesine Karşı Kimyasal Inhibitör Verimliliğin Belirlenmesi

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09:49 min

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April 29, 2020

DOI:

10.3791/60985-v

DOI:
10.3791/60985-v

•

09:49 min
April 29, 2020

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1 Views
, , , , ,
1Department of Biological Sciences,Clemson University, 2Eukaryotic Pathogens Innovation Center,Clemson University

Tags

Toxoplasma GondiiLuciferase-based Growth AssayChemical InhibitorDrug TargetCRISPR-Cas9Intracellular PathogenHigh-throughput ScreeningParasite Doubling TimeHuman Foreskin FibroblastsLuciferase SubstrateMicroplate ReaderNormalized Fold-changeLinear Regression

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Key, M., Bergmann, A., Micchelli, C., Thornton, L. B., Millard, S., Dou, Z. Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay. J. Vis. Exp. (158), e60985, doi:10.3791/60985 (2020).

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