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ルシファーゼベース成長アッセイを用いた細胞内トキソプラズマゴンディ成長に対する化学阻害剤の効率の測定
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
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ルシファーゼベース成長アッセイを用いた細胞内トキソプラズマゴンディ成長に対する化学阻害剤の効率の測定

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09:49 min

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April 29, 2020

DOI:

10.3791/60985-v

DOI:
10.3791/60985-v

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09:49 min
April 29, 2020

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1 Views
, , , , ,
1Department of Biological Sciences,Clemson University, 2Eukaryotic Pathogens Innovation Center,Clemson University

Tags

Toxoplasma GondiiLuciferase-based Growth AssayChemical InhibitorDrug TargetCRISPR-Cas9Intracellular PathogenHigh-throughput ScreeningParasite Doubling TimeHuman Foreskin FibroblastsLuciferase SubstrateMicroplate ReaderNormalized Fold-changeLinear Regression

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Key, M., Bergmann, A., Micchelli, C., Thornton, L. B., Millard, S., Dou, Z. Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay. J. Vis. Exp. (158), e60985, doi:10.3791/60985 (2020).

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