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Immunology and Infection

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JoVE Journal Immunology and Infection
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes
 

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Article DOI: 10.3791/60949-v • 08:52 min • February 5th, 2021
February 5th, 2021 •
1,2, 3, 3, 3
1Department of Pathology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, 2Stony Brook Cancer Center, Stony Brook, NY, 3Department of Medicine, Weill Cornell Medicine, New York, NY

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Here, we provide a detailed description of the procedure to induce colonoscopic-guided pinch biopsies in mice and track wound closure in real time. Additionally, methods for the preparation of tissues for histological, immunohistochemical and molecular analyses of the wound bed are provided.

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Colonoscopic-guided Pinch Biopsies Mice Tissue Changes Colonic Wound Healing Molecular Changes Histologic Changes IBD Pathogenesis Technique Control Location Of Injury Timing Of Wound Healing Process Difficulty Inserting Colonoscope Creating Consistent Wounds Practicing Techniques Visual Demonstration Rigid Bore Endoscope Endoscope Sheath Light Source Video Imaging Device Air Pump Gas Valve Working Channel French Biopsy Forceps Anesthetized Mouse Endoscopic Staging Platform Sedation Level
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