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Umålrettet flydende kromatografi-massespektrometri-baserede metabolomiske analyse af hvedekorn
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1Research and Innovation,Murdoch University, 2Centre for Digital Agriculture,Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre,Murdoch University
Capítulos
- 00:05Introduction
- 01:04Preparation of Grains
- 01:46Metabolite Extraction
- 03:31Preparing Samples for LC-MS Analysis
- 04:10Setting Up Liquid Chromatography Mass Spectrometry
- 05:13Data Processing
- 05:38Results: Biologically Relevant Features Differ Across Wheat Varieties
- 06:42Conclusion
En metode til ikke-målrettet analyse af hvede korn metabolitter og lipider præsenteres. Protokollen indeholder en acetonitril metabolit ekstraktionsmetode og omvendt fase flydende kromatografi-masse spektrometri metode, med erhvervelse i positive og negative elektrospray ionisering tilstande.
