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视觉GPCR-迷你G蛋白信号复合物的战略筛选与表征，成功结晶
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
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视觉GPCR-迷你G蛋白信号复合物的战略筛选与表征，成功结晶

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09:19 min

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March 16, 2020

DOI:

10.3791/60747-v

DOI:
10.3791/60747-v

•

09:19 min
March 16, 2020

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2 Views
, , , , , ,
1Laboratory of Biomolecular Research,Paul Scherrer Institute, 2Department of Biology,ETH Zürich, 3Center for Radiopharmaceutical Sciences,Paul Scherrer Institute, 4Laboratory of Molecular Biology,Medical Research Council

Summary

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本报告描述了不同的洗涤剂的筛选，以准备视觉GPCR，红蛋白，其复合与微型Go。演示了在纯化过程中不同阶段对复杂质量进行特征的生化方法。该协议可以推广为其他膜蛋白复合物，用于其未来的结构研究。

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Cite this Article

Pamula, F., Mühle, J., Blanc, A., Nehmé, R., Edwards, P. C., Tate, C. G., Tsai, C. Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization. J. Vis. Exp. (157), e60747, doi:10.3791/60747 (2020).

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