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Создание высокоспецифических химически индуцированных систем димеризации белка по шагу Phage Выбор комбинаторных однодоменных антител библиотеки
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Bioquímica
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JoVE Journal Bioquímica
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Создание высокоспецифических химически индуцированных систем димеризации белка по шагу Phage Выбор комбинаторных однодоменных антител библиотеки

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10:17 min

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January 14, 2020

DOI:

10.3791/60738-v

DOI:
10.3791/60738-v

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10:17 min
January 14, 2020

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2 Views
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1Department of Biochemistry and Institute for Protein Design,University of Washington

Tags

Protein DimerizationBiosensorPhage DisplaySingle-domain AntibodySmall MoleculeCell BehaviorCell Metabolite MonitoringStreptavidin BeadsBiotinylated LigandNegative And Positive Selection

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Gomez-Castillo, L., Watanabe, K., Jiang, H., Kang, S., Gu, L. Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library. J. Vis. Exp. (155), e60738, doi:10.3791/60738 (2020).

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