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通过旗-生物素串联纯化通过交叉链接和免疫沉淀对蛋白质-RNA相互作用进行转录组-全过程分析
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JoVE Journal Genética
Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
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通过旗-生物素串联纯化通过交叉链接和免疫沉淀对蛋白质-RNA相互作用进行转录组-全过程分析

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08:46 min

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May 18, 2020

DOI:

10.3791/60730-v

DOI:
10.3791/60730-v

•

08:46 min
May 18, 2020

•

3 Views
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1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences,Tsinghua University, 2Department of Molecular Biology,University of Texas Southwestern Medical Center

Summary

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在这里，我们提出了一个改进的 CLIP-seq 协议， 称为 FbioCLIP-seq 与 FLAG 生物素串联纯化，以确定哺乳动物细胞中RNA结合蛋白（RBPs）的RNA靶点。

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Bi, X., Zhang, X., Shen, X. Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification. J. Vis. Exp. (159), e60730, doi:10.3791/60730 (2020).

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