/
/
Isolering, kultur och adipogenic Induktion av neurala Crest Original Fett-Härledda stamceller från periaorektisk fettvävnad
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Isolering, kultur och adipogenic Induktion av neurala Crest Original Fett-Härledda stamceller från periaorektisk fettvävnad
5,849 Views
•
08:31 min
•
March 02, 2020
DOI:
1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
Summary
Automatically generated
Vi presenterar ett protokoll för isolering, kultur och adipogenic induktion av neurala crest härledda fett-härledda stamceller (NCADSCs) från periaortic fettvävnad wnt-1 Cre+/-; Rosa26RFP/+ möss. NCADSCs kan vara en lättillgänglig källa till ADSCs för modellering adipogenesis eller lipogenesis in vitro.
Read Article
Cite this Article
Qi, Y., Miao, X., Xu, L., Fu, M., Peng, S., Shi, K., Li, J., Ye, M., Li, R. Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue. J. Vis. Exp. (157), e60691, doi:10.3791/60691 (2020).
Copy