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Isolement, culture et induction adipogénique des cellules souches adipeuses originales dérivées de l’adipose de l’adipose de l’adiacisation périortique
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1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
Capítulos
- 00:04Introduction
- 01:24Periaortic Adipose Tissue (PAAT) Dissection
- 02:55Stromal Vascular Cell Fraction (SVCF) Isolation
- 04:51Neural Crest Original Adipose-Derived Stem Cell (NCADSC) Culture
- 06:13NCADSC Adipogenic Induction
- 06:42Results: Representative Adipocyte Differentiation and Characterization
- 07:58Conclusion
Nous présentons un protocole pour l’isolement, la culture, et l’induction adipogenic des cellules souches adipeuses dérivées de crête neurale (NCADSCs) du tissu adipeux périortique de Wnt-1 Cre-/-; Rosa26RFP / souris. Les NCADSC peuvent être une source facilement accessible d’ADSCs pour la modélisation de l’adipogénèse ou de la lipogenèse in vitro.
