Journal
/
Neurociência
/
Analyse mitokondriel transport og morfologi i Human Induceret pluripotente stamceller-afledte neuroner i arvelige spastiske paraplegi
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Analyse mitokondriel transport og morfologi i Human Induceret pluripotente stamceller-afledte neuroner i arvelige spastiske paraplegi

DOI:
10.3791/60548-v

•

07:32 min

•

February 09, 2020

•

, , , ,
1Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, 2Department of Bioengineering,University of Illinois at Chicago, 3MD Program,University of Illinois College of Medicine Rockford

Capítulos

  • 00:04Introduction
  • 00:50Telencephalic Glutamatergic Neuron Generation
  • 01:49Time-Lapse Imaging of Mitochondrial Transport Along Telencephalic Glutamatergic Neuron Axons
  • 02:44Cortical Neuron Mitochondrial Transport Analysis
  • 04:17Cortical Neuron Mitochondrial Morphology Analysis
  • 05:32Results: Representative Mitochondrial Transport and Morphology Analyses
  • 06:47Conclusion

Summary

Tadução automática

Tadução automática

Nedsat mitokondrielle transport og morfologi er involveret i forskellige neurodegenerative sygdomme. Den præsenterede protokol bruger inducerede pluripotente stamceller-afledte forhjernen neuroner til at vurdere mitokondrielle transport og morfologi i arvelig spastisk paraplegi. Denne protokol giver mulighed for karakterisering af mitokondriel handel langs axoner og analyse af deres morfologi, som vil lette studiet af neurodegenerative sygdomme.

Tags

Mitochondrial TransportMitochondrial MorphologyHuman Induced Pluripotent Stem Cell-derived NeuronsHereditary Spastic ParaplegiaAxonal DegenerationMitochondrial DynamicsNeurological DiseasesLive Cell ImagingMitochondrial TraffickingTherapeutic TargetsNeurodegenerative DiseasesNeurosphere DifferentiationAxon IdentificationMitochondrial StainingTime-lapse ImagingImageJ Analysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

Effektiv derivering af humane Neuronal progenitorer og neuroner fra Pluripotente humane embryonale stamceller med småmolekyle Induktion

Specifikationen af ​​Telencephalic glutamaterge neuroner fra humane pluripotente stamceller

Analyse Dendritiske Morfologi i Kolonner og Lag

Modellering Zika Virus infektion i udviklingslandene menneskelige hjerne In Vitro ved hjælp af stamceller afledt Cerebral Organoids

Metode for høj hastighed Stretch skade af menneskelig induceret pluripotente stamceller-afledt neuroner i en 96-brønds Format

Opdeling af humane stamcelle-afledte neuroner inden for præ-monterede plastik Microfluidic chips

Post-differentiering Replating af humane pluripotente stamcelle-afledte neuroner for højt indhold screening af Neuritogenesis og synapse modning

Omdannelse af humane induceret pluripotente stamceller (iPSCs) til funktionel spinal og kraniel motor neuroner brug PiggyBac vektorer

Migration, Chemo-Attraction, og Co-Kultur Assays for Human Stem Cell-Afledt endotelceller og GABAergic Neurons

Read Article