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使用消耗法对溶液分散无机纳米颗粒进行短肽吸附研究
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JoVE Journal Química
Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
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使用消耗法对溶液分散无机纳米颗粒进行短肽吸附研究

DOI:
10.3791/60526-v

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09:43 min

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April 11, 2020

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, , , ,
1Nanotechnology Education and Research Center,South Ural State University, 2Laboratory of Computational Modelling of Drugs,South Ural State University

Capítulos

  • 00:00Introduction
  • 00:58Preparation of 16 mM Dipeptide Stock Solution
  • 02:18Preparation of Peptide Dilutions
  • 03:25Preparation of Titania Sole
  • 04:26Mixing and Thermostating
  • 05:32Filtration of Thermostated Samples
  • 06:10Preparation of Derivatization Solutions
  • 06:58Derivatization
  • 07:37HPLC Analysis
  • 07:58Resultados
  • 09:05Conclusion

Summary

Tadução automática

Tadução automática

理解生物分子-无机固相相互作用的第一步是揭示基本的物理化学常数，可以通过建立吸附等体来评估这些常数。液相的吸附受受动力学、表面容量、pH 和竞争吸附的限制，在设置吸附实验之前，应谨慎考虑这些。

Tags

Peptide AdsorptionInorganic NanoparticlesDepletion MethodAdsorption IsothermsDipeptide AdsorptionTitanium DioxidePH AdjustmentMES BufferSolution PreparationConcentration Dilution

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