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Isolation und 3D Kollagen Sandwich Kultur der primären Maus Hepatozyten, um die Rolle des Zytoskeletts in Bile Canalicular Formation In Vitro zu studieren
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
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Isolation und 3D Kollagen Sandwich Kultur der primären Maus Hepatozyten, um die Rolle des Zytoskeletts in Bile Canalicular Formation In Vitro zu studieren

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10:12 min

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December 20, 2019

DOI:

10.3791/60507-v

DOI:
10.3791/60507-v

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10:12 min
December 20, 2019

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9 Views
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1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

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3D Collagen Sandwich CulturePrimary Mouse HepatocytesBile Canalicular FormationCytoskeletonLiver IsolationHepatocyte IsolationIn Vitro StudiesPerfusionHeparinDMEMCollagen IPBSCell Culture

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Korelova, K., Jirouskova, M., Sarnova, L., Gregor, M. Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro. J. Vis. Exp. (154), e60507, doi:10.3791/60507 (2019).

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