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Isolation og 3D kollagen sandwich kultur af primære mus hepatocytter at studere rollen af cytoskelet i galde Canalicular dannelse in vitro

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Isolation og 3D kollagen sandwich kultur af primære mus hepatocytter at studere rollen af cytoskelet i galde Canalicular dannelse in vitro

10,684 Views

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10:12 min

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December 20, 2019

DOI:

10.3791/60507-v

DOI:

10.3791/60507-v

•

10:12 min

December 20, 2019

•

9 Views

Katerina Korelova, Marketa Jirouskova, Lenka Sarnova, Martin Gregor

¹Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Tags

3D Collagen Sandwich Culture Primary Mouse Hepatocytes Bile Canalicular Formation Cytoskeleton Liver Isolation Hepatocyte Isolation In Vitro Studies Perfusion Heparin DMEM Collagen I PBS Cell Culture

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