Journal
/
Bioquímica
/
Fluxo de trabalho de espectrometria de massa de imunoprecipitação sem rótulos para perfis de interactom nuclear em larga escala
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Fluxo de trabalho de espectrometria de massa de imunoprecipitação sem rótulos para perfis de interactom nuclear em larga escala

15,195 Views

•

11:19 min

•

November 17, 2019

DOI:

10.3791/60432-v

DOI:
10.3791/60432-v

•

11:19 min
November 17, 2019

•

10 Views
, ,
1Department of Molecular, Cellular and Developmental Biology,University of Colorado, 2Linda Crnic Institute for Down Syndrome,University of Colorado School of Medicine

Tags

Label-freeImmunoprecipitationMass SpectrometryNuclear InteractomeProtein-protein InteractionsSubcellular FractionationAffinity PurificationProteomics WorkflowCell LysisNuclear LysateDounce Homogenization

Read Article

Cite this Article

Guard, S. E., Ebmeier, C. C., Old, W. M. Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling. J. Vis. Exp. (153), e60432, doi:10.3791/60432 (2019).

Download .ris file

Copy

Share Video

.

Copy