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用于大规模核相互作用分析的无标签免疫沉淀质谱工作流程
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
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用于大规模核相互作用分析的无标签免疫沉淀质谱工作流程

15,195 Views

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11:19 min

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November 17, 2019

DOI:

10.3791/60432-v

DOI:
10.3791/60432-v

•

11:19 min
November 17, 2019

•

10 Views
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1Department of Molecular, Cellular and Developmental Biology,University of Colorado, 2Linda Crnic Institute for Down Syndrome,University of Colorado School of Medicine

Tags

Label-freeImmunoprecipitationMass SpectrometryNuclear InteractomeProtein-protein InteractionsSubcellular FractionationAffinity PurificationProteomics WorkflowCell LysisNuclear LysateDounce Homogenization

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Guard, S. E., Ebmeier, C. C., Old, W. M. Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling. J. Vis. Exp. (153), e60432, doi:10.3791/60432 (2019).

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