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Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
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Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
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March 18, 2020
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We outline a 9-day protocol for the transduction and expansion of rhesus macaque peripheral blood mononuclear cells which yields cells with excellent co-expression of the genes of interest in sufficient number for infusion studies of cell efficacy.
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Pampusch, M. S., Skinner, P. J. Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype. J. Vis. Exp. (157), e60400, doi:10.3791/60400 (2020).
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