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解剖果蝇黑色素组飞行肌肉为奥米克斯方法

JoVE Journal
Genética

This content is Free Access.

JoVE Journal Genética

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

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解剖果蝇黑色素组飞行肌肉为奥米克斯方法

DOI:

10.3791/60309-v

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08:33 min

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October 17, 2019

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Shao-Yen Kao*¹, Elena Nikonova*¹, Keshika Ravichandran*¹, Maria L. Spletter²

¹Biomedical Center, Department of Physiological Chemistry,Ludwig-Maximilians-University Munich, ²Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry,Ludwig-Maximilians-University Munich

Capítulos

00:04Título
00:51Pupae Staging
01:28Indirect Flight Muscle (IFM) Dissection Before 48 Hours After Puparium Formation (APF)
03:20IFM Dissection After 48 Hours APF
06:04Results: Representative Application of IFM Dissections to Investigate Bruno1 Function in Alternative Splicing
07:40Conclusion

Summary

Tadução automática

果蝇飞行肌肉是研究转录调节、替代拼接、代谢和肌生物学的有力模型。我们提出了从活pupae中解剖荧光标记飞行肌肉的协议，以生成高富集的样品，非常适合蛋白质组学和深度测序。这些样本可以提供重要的机械见解，肌肉发育的各个方面。

Tags

Keywords: Drosophila Melanogaster Flight Muscles Dissection Omics Approaches GFP-labeled Indirect Flight Muscle Pupae Development Gene Expression Protein Expression RT-PCR Western Blot Staging Sexing Fluorescent Microscopy Forceps PBS

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