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Quantification of Coenzyme A in Cells and Tissues
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September 27, 2019
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This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.
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Frank, M. W., Subramanian, C., Rock, C. O., Jackowski, S. Quantification of Coenzyme A in Cells and Tissues. J. Vis. Exp. (151), e60182, doi:10.3791/60182 (2019).
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