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骨髓移植平台研究树突状细胞在移植与宿主疾病中的作用
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
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骨髓移植平台研究树突状细胞在移植与宿主疾病中的作用

DOI:
10.3791/60083-v

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08:05 min

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March 17, 2020

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, , , , , , , ,
1Cancer Division, Burnett School of Biomedical Sciences,University of Central Florida, 2Department of Biochemistry,Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences,Houston Methodist Research Institute, 4Department of Epidemiology,University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences,University of Pittsburgh Medical Center, Hillman Cancer Center

Capítulos

  • 00:04Introduction
  • 00:49Graft-Versus-Host Disease (GVHD) Induction
  • 02:53Donor T Cell Preparation
  • 04:54Bone Marrow-Derived Dendritic Cell (BM-DC) Preparation
  • 05:58Results: Representative Effects of Dendritic Cell Function in GVHD
  • 07:26Conclusion

Summary

Tadução automática

Tadução automática

移植与宿主疾病是异体骨髓移植后的主要并发症。树突状细胞在移植与宿主疾病的发病机制中起着关键作用。本文介绍了一种新型的骨髓移植平台，用于研究树突状细胞在移植与宿主疾病发展中的作用以及移植对白血病的影响。

Tags

Bone Marrow TransplantationDendritic CellsGraft-versus-host DiseaseT-cell DepletionCell IsolationCell CultureImmune Cell PopulationsMacrophagesNeutrophilsCell RegenerationBone Marrow PreparationCell HarvestingCell StrainingCell WashingCell ResuspensionCell IncubationCell CountingCell Pooling

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