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Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

DOI:
10.3791/60048-v

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08:43 min

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January 31, 2020

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, , , , , , , ,
1Medical School of Hebei University, 2Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province

Capítulos

  • 00:04Introduction
  • 01:15Constructing the Disease Model
  • 02:32Obtaining iNKT (Invariant Natural Killer T) Cells with Adoptive Cellular Therapy
  • 06:20Results: Changes in the Mice
  • 07:25Conclusion

Summary

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This protocol uses G6PI mixed peptides to construct rheumatoid arthritis models that are closer to that of human rheumatoid arthritis in CD4+ T cells and cytokines. High purity invariant natural killer T cells (mainly iNKT2) with specific phenotypes and functions were obtained by in vivo induction and in vitro purification for adoptive immunotherapy.

Tags

Adoptive ImmunotherapyINKT CellsGlucose-6-phosphate Isomerase (G6PI)Rheumatoid Arthritis (RA) ModelRA PathogenesisT CellsB CellsInnate ImmunityIn VivoEmulsificationPertussis ToxinSpleenCell IsolationLymphocyte SeparationINKT Cell Purification

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