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单分子Fürster共振能量传递方法，用于GEN1对霍利迪结分辨率进行实时调查

JoVE Journal
Genética

This content is Free Access.

JoVE Journal Genética

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

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单分子Fürster共振能量传递方法，用于GEN1对霍利迪结分辨率进行实时调查

9,025 Views

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11:27 min

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September 18, 2019

DOI:

10.3791/60045-v

DOI:

10.3791/60045-v

•

11:27 min

September 18, 2019

•

14 Views

Mohamed A. Sobhy*¹, Amer Bralić*¹, Vlad-Stefan Raducanu¹, Muhammad Tehseen¹, Yujing Ouyang¹, Masateru Takahashi¹, Fahad Rashid¹, Manal S. Zaher¹, Samir M. Hamdan¹

¹Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

Summary

这里介绍的是一个执行单分子Fürster共振能量转移以研究HJ分辨率的协议。双色交替激励用于确定解离常数。然后，在实时裂解测定中应用单色时移smFRET，以获得HJ分辨率前的沉思时间分布。

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