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CRISPR/Cas9 시스템을 사용하여 트리파노소마 크루지의 아마스티고테 스테이지에 유전자 녹아웃 직접 도입
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JoVE Journal Genética
Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
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CRISPR/Cas9 시스템을 사용하여 트리파노소마 크루지의 아마스티고테 스테이지에 유전자 녹아웃 직접 도입

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06:13 min

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July 31, 2019

DOI:

10.3791/59962-v

DOI:
10.3791/59962-v

•

06:13 min
July 31, 2019

•

2 Views
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1Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology (AIST)

Tags

Gene KnockoutCRISPR/Cas9Trypanosoma CruziAmastigoteHost Cell InfectionParasitologyExtracellular AmastigotesTrypomastigotesCell CultureElectroporation

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Akutsu, Y., Doi, M., Furukawa, K., Takagi, Y. Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System. J. Vis. Exp. (149), e59962, doi:10.3791/59962 (2019).

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