Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Journal

Biologia do Desenvolvimento

JoVE Journal
Biologia do Desenvolvimento

This content is Free Access.

JoVE Journal Biologia do Desenvolvimento

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

DOI:

10.3791/59956-v

•

05:41 min

•

January 28, 2020

•

Tomasz Dobrzycki*^1,2, Monika Krecsmarik*^1,2, Rui Monteiro^2,3

¹MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital,University of Oxford, ²BHF Centre of Research Excellence, ³Institute of Cancer and Genomic Sciences,University of Birmingham

Capítulos

00:04Título
00:40ISH-stained Embryo Imaging and DNA Extraction
02:26Image Analysis with Fiji Software
03:53Results: Parallel Image Quantitation and Genotyping Protocol
05:13Conclusion

Summary

Tadução automática

Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ hybridization.

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Wholemount In Situ Hybridization for Astyanax Embryos

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Read Article