Journal
/
Bioquímica
/
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

7,745 Views

•

09:24 min

•

January 30, 2020

DOI:

10.3791/59914-v

DOI:
10.3791/59914-v

•

09:24 min
January 30, 2020

•

1 Views
,
1Committee on Cancer Biology,The University of Chicago, 2Pritzker School of Molecular Engineering,The University of Chicago

Summary

Automatically generated

The goal of this protocol is to show how to use Lattice Light-Sheet Microscopy to four-dimensionally visualize surface receptor dynamics in live cells. Here T cell receptors on CD4+ primary T cells are shown.

Explore More Videos

Four-dimensional ImagingLattice Light-sheet MicroscopyLive Cell ImagingReal-time TrackingMolecular DynamicsT-cell ReceptorAntigen-presenting CellsSample PreparationCell Labeling

Read Article

Cite this Article

Rosenberg, J., Huang, J. Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy. J. Vis. Exp. (155), e59914, doi:10.3791/59914 (2020).

Download .ris file

Copy

Share Video

.

Copy