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Biologia do Desenvolvimento

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在无进料和异种定义的条件下,从人类多能干细胞中血性内皮物分化

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

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在无进料和异种定义的条件下,从人类多能干细胞中血性内皮物分化

DOI:

10.3791/59823-v

•

09:00 min

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June 16, 2019

•

Ryo Ohta*¹, Ryohichi Sugimura*¹, Akira Niwa, Megumu K. Saito

¹Center for iPS cell research and application (CiRA)

Capítulos

00:04Título
01:11Pluripotent Stem Cell Colony Formation
02:45Hemogenic Induction and Isolation of Hemogenic Endothelium
04:08Induction of Endothelial-to-hematopoietic Transition (EHT)
05:00Flow Cytometry Analysis of Hematopoietic Cells
06:27Colony Forming Unit Assay of Hematopoietic Cells
07:30Results: Hemogenic Induction
08:19Conclusion

Summary

Tadução automática

在这里,我们提出了一个协议,以精确形成血源内皮,从人类多能干细胞在馈送和异种条件下。该方法在治疗性化合物的疾病建模和筛选中有着广泛的应用。

Tags

Keywords: Hemogenic Endothelium Pluripotent Stem Cells Feeder-free Xeno-free Blood Cells Hematology Immunology Genetic Intervention Gene Editing LM511-E8 PSC Colonies Dissociation Buffer Differentiation Hypoxia Culture Conditions

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