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Real-tempo, semi-automatizado de medição fluorescente do pH líquido de superfície das vias aéreas das células epiteliais das vias aéreas humanas primárias
This content is Free Access.
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Capítulos
- 00:04Título
- 01:09Primary Human Airway Epithelial Cell (hAEC) Preparation
- 01:53Background Measurement
- 04:43Kinetics Measurement
- 05:45In Situ pH Calibration
- 06:28Data Analysis
- 07:58Results: Representative pH Calibration in Primary hAECs In Situ
- 09:34Conclusion
Tags
Keywords:
Real-timeSemi-automatedFluorescent MeasurementAirway Surface Liquid PHPrimary Human Airway Epithelial CellsIon ChannelsTransportersExtracellular PH RegulationCystic FibrosisAsthmaCOPDAir-liquid InterfaceBicarbonate BufferPlate ReaderTemperature ControlCarbon Dioxide ControlHumidity CassetteKinetic Loop
