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Purification of a High Molecular Mass Protein in Streptococcus mutans
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Purification of a High Molecular Mass Protein in Streptococcus mutans

Purification of a High Molecular Mass Protein in Streptococcus mutans

DOI:
10.3791/59804-v

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09:51 min

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September 14, 2019

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, , , ,
1Department of Translational Research,Tsurumi University School of Dental Medicine, 2Laboratory for Oral Health Science

Capítulos

  • 00:06Título
  • 00:51PCR Amplification
  • 02:38Cell Transformation
  • 03:35Purification of Polyhistidine-tagged GTF-SI
  • 07:29Results: Generation of S. mutans His-gtfC and Functional Restoration
  • 08:59Conclusion

Summary

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Tadução automática

Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.

Tags

Protein PurificationStreptococcus MutansHigh Molecular Mass ProteinPCRGenomic DNA ExtractionGel ElectrophoresisGel ExtractionNested PCRElectroporationCompetent CellsBrain-heart Infusion Broth

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