Journal
/
Imunologia e Infecção
/
Количественная флуоресценция в ситу Гибридизации (FISH) и иммунофлуоресценции (ИФ) специфических генных продуктов в KSHV-зараженных клетках
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
Please note that all translations are automatically generated. Click here for the English version.

Количественная флуоресценция в ситу Гибридизации (FISH) и иммунофлуоресценции (ИФ) специфических генных продуктов в KSHV-зараженных клетках

11,855 Views

•

00:06 min

•

August 27, 2019

DOI:

10.3791/59697-v

DOI:
10.3791/59697-v

•

00:06 min
August 27, 2019

•

9 Views
,
1Norfolk Academy, 2Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute,Yale School of Medicine

Tags

Quantitative FISHImmunofluorescenceKSHVHost-virus InteractionsViral Gene ProductsRNAProteinBiochemical AssaysCell CultureFixationPermeabilizationBlockingPrimary AntibodySecondary AntibodyFluorescent LabelingHybridizationOligonucleotidesDenaturation

Read Article

Cite this Article

Vallery, T. K., Steitz, J. A. Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells. J. Vis. Exp. (150), e59697, doi:10.3791/59697 (2019).

Download .ris file

Copy

Share Video

.

Copy