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翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA

JoVE Journal
Bioquímica
Author Produced

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JoVE Journal Bioquímica

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

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翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA

DOI:

10.3791/59624-v

•

08:53 min

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May 25, 2019

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Patrick Moran², Yichen Guo⁴, Rong Yuan³, Nicholas Barnekow, Jordan Palmer, Adam Beck, Bin Ren^4,5

¹Blood Research Institute,Blood Center of Wisconsin, ²Department of Medicine,Medical College of Wisconsin, ³Department of Surgery,The University of Alabama at Birmingham, ⁴Department of Biomedical Engineering,The University of Alabama at Birmingham, ⁵GBS Program, Graduate School,The University of Alabama at Birmingham

Capítulos

00:00Título
02:34Methods
07:06Resultados
07:39Conclusion

Summary

Tadução automática

我们通过结合RNA纯化结合的增强绿色荧光蛋白(EGFP)的EC特异性遗传标记,直接在小鼠脑、肺和心脏组织中直接从小鼠脑、肺和心脏组织中从血管内皮细胞(EC)中纯化核糖体结合体mRNA的mRNA。.

Tags

RNA Isolation Endothelial Cells Ribosome Affinity Purification (TRAP)Transgenic Mice GFP-tagged Ribosomes Transcriptome Analysis Angiogenesis Epigenetics Transcription Regulation Genotyping RNA Sequencing RT-qPCR

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