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Skræddersy in vivo cytotoksiske assays til at studere immun dominans i tumor specifikke CD8+ T- celle respons
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Skræddersy in vivo cytotoksiske assays til at studere immun dominans i tumor specifikke CD8+ T- celle respons

DOI:
10.3791/59531-v

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10:13 min

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May 06, 2019

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, ,
1Department of Microbiology and Immunology,Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy,Western University, 3Department of Surgery, Division of General Surgery,Western University, 4Lawson Health Research Institute

Capítulos

  • 00:04Título
  • 01:14T Antigen (Ag)-Expressing Tumor Cell Inoculation
  • 02:29Target Splenocyte Preparation
  • 04:10Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
  • 05:44CFSE-Labeling Evaluation
  • 06:50CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
  • 07:31Data Acquisition
  • 08:21Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
  • 09:36Conclusion

Summary

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Tadução automática

Vi beskriver her en flow cytometri-baseret in vivo drab assay, der muliggør undersøgelse af immun dominans i cytotoksiske T-lymfocyt (CTL) svar til en model tumor antigen. Vi giver eksempler på, hvordan denne elegante analyse kan anvendes til Mekanistiske undersøgelser og til Drug effektivitet test.

Tags

Keywords: In Vivo Cytotoxicity AssayTumor-specific CD8+ T Cell ResponsesImmunodominanceTumor AntigensCytotoxic T Cell ResponsesNK CellsInnate-like T LymphocytesAdherent Tumor Cell LineTarget Splenocyte PreparationC57BL/6 Mice

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