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活体细胞毒性试验研究肿瘤特异性 CD8+ t 细胞反应中的免疫调节
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
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活体细胞毒性试验研究肿瘤特异性 CD8+ t 细胞反应中的免疫调节

DOI:
10.3791/59531-v

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10:13 min

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May 06, 2019

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, ,
1Department of Microbiology and Immunology,Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy,Western University, 3Department of Surgery, Division of General Surgery,Western University, 4Lawson Health Research Institute

Capítulos

  • 00:04Título
  • 01:14T Antigen (Ag)-Expressing Tumor Cell Inoculation
  • 02:29Target Splenocyte Preparation
  • 04:10Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
  • 05:44CFSE-Labeling Evaluation
  • 06:50CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
  • 07:31Data Acquisition
  • 08:21Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
  • 09:36Conclusion

Summary

Tadução automática

Tadução automática

我们这里描述了一种基于流式细胞仪的体内杀伤检测方法, 该方法能够检查细胞毒性 T 淋巴细胞 (CTL) 对模型肿瘤抗原的反应中的免疫调节。我们提供了如何将这种优雅的检测方法用于机械研究和药物疗效测试的示例。

Tags

Keywords: In Vivo Cytotoxicity AssayTumor-specific CD8+ T Cell ResponsesImmunodominanceTumor AntigensCytotoxic T Cell ResponsesNK CellsInnate-like T LymphocytesAdherent Tumor Cell LineTarget Splenocyte PreparationC57BL/6 Mice

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