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Ячейки бесплатно белка выражение с использованием быстро растущих бактерией Vibrio natriegens
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Bioengenharia
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JoVE Journal Bioengenharia
Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens
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Ячейки бесплатно белка выражение с использованием быстро растущих бактерией Vibrio natriegens

16,744 Views

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08:52 min

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March 14, 2019

DOI:

10.3791/59495-v

DOI:
10.3791/59495-v

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08:52 min
March 14, 2019

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12 Views
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1Department of Genetics,Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering

Tags

Cell-free Protein ExpressionVibrio NatriegensCrude ExtractSonication-centrifugationBioproductionSynthetic BiologyLB-V2 MediaOD 600S30 Lysis BufferSonication

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Wiegand, D. J., Lee, H. H., Ostrov, N., Church, G. M. Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens. J. Vis. Exp. (145), e59495, doi:10.3791/59495 (2019).

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