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Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
 

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development

Article DOI: 10.3791/59481-v • 09:10 min • June 25th, 2019
June 25th, 2019 •
1,2,3,4, 1,2,3,4
1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona

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Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.

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3-D Collagen-based Hydrogels Axonal Growth Behavior Nervous System Development Molecules Axonal Guidance Neuronal Migration Analysis Quick And Simple Complex Software Training Mirian Segura-Feliu Technician Plate Cells Petri Dishes Incubate Culture Medium Transfection DNA Encoding Liposome-based Transfection Method Serum-free Medium Liposomal Tube
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