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在Vivo直接重新编程居民胶质细胞到内神经通过病毒载体的脑内注射

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

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在Vivo直接重新编程居民胶质细胞到内神经通过病毒载体的脑内注射

DOI:

10.3791/59465-v

•

12:26 min

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June 17, 2019

•

Maria Pereira, Marcella Birtele, Daniella Rylander Ottosson

¹Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC,Lund University

Capítulos

00:04Título
01:16AAV5 Viral Vector Production
03:41AAV5 Viral Vector Purification
06:01Injection of Reprogramming Factors into the Brain
08:34Electrophysiological Recordings
10:33Results: In Vivo Reprogrammed Neurons Are Functional and Show Maturation Over Time
11:44Conclusion

Summary

Tadução automática

该协议旨在利用大脑中基于AAV的病毒系统和FLEX突触素驱动的GFP报告器,在体内直接重新编程的内神经,从而在体内进行细胞识别和进一步分析。

Tags

AI-generated Neurons In Vivo Reprogramming NG2-glia Parvalbumin-positive Interneurons AAV Viral Vector HEK293T Cell Transfection Iodixanol Gradient Ultracentrifugation

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