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Evaluering af Synaptic mangfoldighed ved hjælp af hele-celle Patch-klemme Elektrofysiologi
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Neurociência
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JoVE Journal Neurociência
Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

Evaluering af Synaptic mangfoldighed ved hjælp af hele-celle Patch-klemme Elektrofysiologi

DOI:
10.3791/59461-v

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10:52 min

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April 23, 2019

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,
1Neuroscience Program, Schulich School of Medicine and Dentistry,University of Western Ontario, 2Robarts Research Institute, Schulich School of Medicine and Dentistry,University of Western Ontario, 3Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario

Capítulos

  • 00:04Título
  • 00:49Obtain the Whole-cell Configuration
  • 02:34Experiment 1
  • 04:13Experiment 2
  • 05:11Experiment 3
  • 06:47Experiment 4
  • 07:59Data Analysis
  • 08:53Results: 4-AP Application and High-frequency Stimulation Reveal Synaptic Multiplicity
  • 10:19Conclusion

Summary

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Tadução automática

Vi præsenterer her, en protokol for at vurdere den funktionelle synaptic mangfoldighed ved hjælp af hele-celle patch klemme Elektrofysiologi i akut hjernen skiver.

Tags

Keywords: Synaptic MultiplicityWhole-cell Patch-clamp ElectrophysiologyAction PotentialNeurotransmitter ReleasePostsynaptic CurrentTTXCadmiumEPSCLow-calcium ACSF

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