Journal
/
Biologia do Desenvolvimento
/
解冻、培养和冷冻保存的嗜酸性粒细胞系
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
Please note that all translations are automatically generated. Click here for the English version.

解冻、培养和冷冻保存的嗜酸性粒细胞系

DOI:
10.3791/59459-v

•

07:57 min

•

April 16, 2019

•

, , ,
1Drosophila Genomics Resource Center, Department of Biology,Indiana University Bloomington

Capítulos

  • 00:04Título
  • 00:21Frozen Drosophila Cell Line Thawing and Reviving
  • 02:01Semi-adherent Cell Subculture
  • 03:26Adherent Cells Detachment
  • 04:33Manual Cell Counting
  • 05:23Drosophila Cell Line Cryopreservation
  • 06:41Results: Representative Images of Distinct Drosophila Cell Lines
  • 07:30Conclusion

Summary

Tadução automática

Tadução automática

食子细胞系是基础和生物医学研究的重要试剂。本文提供了解冻, 亚培养, 冷冻保存常用的嗜食子细胞系, 以协助研究人员将这些试剂的使用纳入他们的研究。

Tags

Drosophila Cell LinesThawingCulturingCryopreservingCell CultureCell SuspensionCell DensityCell ProliferationCell ViabilityCell MorphologyLaminar Flow HoodCell Culture MediaCentrifugationCell PassageMicroorganismal Contamination

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

的测定血细胞种群<em>果蝇</em>幼虫

建立基因组编辑的人多能干细胞系：从定位到隔离

培养人多能性和神经干细胞的基本和临床前研究一个封闭的细胞培养系统

从小鼠胚胎植入前胚胎干细胞系的推导

果蝇蛹卵巢的解剖与染色

基于基因组编辑的果蝇组织特异二进制转录系统的高效生成策略

体外检测评估神仙体头三个月滋养细胞细胞系的迁移、入侵和增殖

胎儿和新生儿小鼠长骨的培养与测量

基因组编辑细胞系细胞融合，用于细胞结构和功能的扰动

Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization

Read Article