This content is Free Access.
多点PCR和毛细血管电泳对鱼类致病菌的多点变量数串联重复分析
10,460 Views
•
10:33 min
•
June 17, 2019
DOI:
Summary
Automatically generated
这里介绍的多位数可变数串联重复分析(MLVA)测定使鱼类致病菌Yersinia ruckeri的廉价、坚固和便携式高分辨率基因分型成为可能。从纯培养基开始,该测定采用多路PCR和毛细管电泳,为下游应用生产十位MLVA型材。
Read Article
Cite this Article
Gulla, S., Mohammad, S. N., Colquhoun, D. J. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (148), e59455, doi:10.3791/59455 (2019).
Copy