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DREADD控制神经活动慢性操作的非侵入性策略

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity

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DREADD控制神经活动慢性操作的非侵入性策略

DOI:

10.3791/59439-v

•

00:08 min

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August 25, 2019

•

Jesse Zhan, Ruchi Komal, William T. Keenan, Samer Hattar, Diego C. Fernandez

¹Section on Light and Circadian Rhythms (SLCR),National Institute of Mental Health, ²Howard Hughes Medical Institute, Department of Neuroscience,Scripps Research Institute

Capítulos

00:04Título
00:53Hippocampal Adeno-associated Virus Injection
02:10Repetitive Clozapine-N-Oxide (CNO) Delivery
03:20Chronic CNO Treatment
04:07Restricted CNO Treatment
05:01Data Analysis
06:15Results: Representative Effects of Repetitive and Chronic CNO Delivery
07:40Conclusion

Summary

Tadução automática

在这里，我们描述了两种非侵入性方法，使用小鼠的化学遗传学来长期控制神经元活动。眼药水用于每天提供氯扎平-N-氧化物（CNO）。我们还描述了在饮用水中长期使用CNO的两种方法。这些慢性神经元控制策略需要最少的干预，以减少动物的压力。

Tags

Keywords: DREADD Chemogenetics CNO Non-invasive Chronic Repetitive Eyedrop Drinking Water Stereotaxic Surgery Hippocampus AAV Analgesia Handling Acclimation Dosage

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