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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
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July 25, 2019
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Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.
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Masclef, L., Maxime, U., Ahmed, O., Sen Nkwe, N., Barbour, H., Iannantuono, N. V., Boubekeur, A., Daou, S., Affar, E. B. In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones. J. Vis. Exp. (149), e59385, doi:10.3791/59385 (2019).
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