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In Vivo Calcium Imaging in C. elegans Body Wall Muscles
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JoVE Journal Bioquímica
In Vivo Calcium Imaging in C. elegans Body Wall Muscles

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

7,420 Views

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08:03 min

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October 20, 2019

DOI:

10.3791/59175-v

DOI:
10.3791/59175-v

•

08:03 min
October 20, 2019

•

7 Views
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1Department of Biological Sciences,University of Illinois at Chicago, 2Department of Integrative Biology and Physiology,University of Minnesota, 3Department of Anatomy and Cell Biology,University of Illinois at Chicago

Summary

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This method provides a way to couple optogenetics and genetically encoded calcium sensors to image baseline cytosolic calcium levels and changes in evoked calcium transients in the body wall muscles of the model organism C. elegans.

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Cite this Article

Martin, A. A., Alford, S., Richmond, J. E. In Vivo Calcium Imaging in C. elegans Body Wall Muscles. J. Vis. Exp. (152), e59175, doi:10.3791/59175 (2019).

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