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Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay

DOI:
10.3791/59014-v

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05:45 min

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April 05, 2019

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, ,
1Department of Neurology and Neuroscience Center,The First Hospital of Jilin University

Capítulos

  • 00:04Título
  • 00:42Sample Preparation and Incubation
  • 02:27Secondary Antibody Incubation and Mounting
  • 03:34Results: Human Serum Anti-AQP4 Antibody Detection by CBA
  • 05:08Conclusion

Summary

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Tadução automática

Cell-based assay is a widely used method to detect serum anti-aquaporin-4 immunoglobulin G. This method could be applied to clinical diagnosis and scientific researches of neuromyelitis optical spectrum disorders.

Tags

Anti-AQP4 IgGCell-based AssayNeuromyelitis Optica Spectrum DisordersClinical DiagnosisScientific StudiesSerum SamplesBiochip SlidePBS Wash BufferTWEEN 20Positive ControlNegative ControlFluorescent Secondary AntibodyEmbedding MediumCover Glass

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