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Rab10リン酸化を評価してパーキンソン病関連LRRK2キナーゼ経路を尋問するためのヒト末梢血好中球分離
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12:49 min
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March 21, 2020
DOI:
1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2The Michael J. Fox Foundation for Parkinson’s Research, 3Department of Medical and Molecular Genetics,Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson’s Disease, Toronto Western Hospital,University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee
Summary
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ロイシンの変異は、リピートキナーゼ2遺伝子(LRRK2)が遺伝性パーキンソン病を引き起こす。我々はヒト末梢血中のRab10のLRRK2制御リン酸化を評価するための容易で、強い方法を開発した。これは、LRRK2キナーゼ経路活性の増加を有する個体を同定するのに役立つ可能性がある。
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Fan, Y., Tonelli, F., Padmanabhan, S., Baptista, M. A., Riley, L., Smith, D., Marras, C., Howden, A., Alessi, D. R., Sammler, E. Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation. J. Vis. Exp. (157), e58956, doi:10.3791/58956 (2020).
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